#9 Michael Tessler and Christopher Mason on 16S amplicon vs shotgun sequencing

Michael Tessler
and
Christopher Mason
join me to talk about their comparison of
16S amplicon sequencing and
shotgun sequencing for quantifying microbial diversity.





Links:


The 2017 Nature paper that we discuss: Large-scale differences in microbial biodiversity discovery between 16S amplicon and shotgun sequencing

Michael’s et al. 2016 paper that describes their original 16S study: A Global eDNA Comparison of Freshwater Bacterioplankton Assemblages Focusing on Large-River Floodplain Lakes of Brazil


The sequencing data for these studies is available from NCBI:
PRJNA310230
(16S),
PRJNA389803
(shotgun)

Michael’s website

Christopher’s lab website

The Integrative Biology & Medicine
conference, featuring the talk on Postmodern Synthesis by Eugene Koonin





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