Spatio-Temporal Alterations in Chromosome Dynamics (Jane Skok)

In this episode of the Epigenetics Podcast, we caught up with Jane Skok from New York University School of Medicine to talk about her work on spatio-temporal alterations in chromosome dynamics.
Studies demonstrating that nuclear organization and long-range chromatin interactions play essential roles in gene regulation have been the focus of the Skok Lab, where the team has played a leading role. Their initial studies focused on lymphocyte development and the control of V(D)J recombination, a key part of generating the diverse repertoire of B-cell antibodies and T-cell receptors. The Skok Lab was among the first to demonstrate the possibility of chromatin forming dynamic loops which lead to the formation of reversible intra-locus loops in the immunoglobulin and T-cell receptor loci and to a profound impact on the ability of B and T cells to generate receptor diversity.
 
References

Roldán, E., Fuxa, M., Chong, W., Martinez, D., Novatchkova, M., Busslinger, M., & Skok, J. A. (2005). Locus “decontraction” and centromeric recruitment contribute to allelic exclusion of the immunoglobulin heavy-chain gene. Nature Immunology, 6(1), 31–41. https://doi.org/10.1038/ni1150


Skok, J. A. (2014). Taking a break from the lab: Can it really be done? Trends in Cell Biology, 24(12), 725–726. https://doi.org/10.1016/j.tcb.2014.09.002


Proudhon, C., Snetkova, V., Raviram, R., Lobry, C., Badri, S., Jiang, T., Hao, B., Trimarchi, T., Kluger, Y., Aifantis, I., Bonneau, R., & Skok, J. A. (2016). Active and Inactive Enhancers Cooperate to Exert Localized and Long-Range Control of Gene Regulation. Cell Reports, 15(10), 2159–2169. https://doi.org/10.1016/j.celrep.2016.04.087


Lhoumaud, P., Sethia, G., Izzo, F., Sakellaropoulos, T., Snetkova, V., Vidal, S., Badri, S., Cornwell, M., Di Giammartino, D. C., Kim, K.-T., Apostolou, E., Stadtfeld, M., Landau, D. A., & Skok, J. (2019). EpiMethylTag: Simultaneous detection of ATAC-seq or ChIP-seq signals with DNA methylation. Genome Biology, 20(1), 248. https://doi.org/10.1186/s13059-019-1853-6


Nishana, M., Ha, C., Rodriguez-Hernaez, J., Ranjbaran, A., Chio, E., Nora, E. P., Badri, S. B., Kloetgen, A., Bruneau, B. G., Tsirigos, A., & Skok, J. A. (2020). Defining the relative and combined contribution of CTCF and CTCFL to genomic regulation. Genome Biology, 21(1), 108. https://doi.org/10.1186/s13059-020-02024-0
 


Related Episodes

Identification of Functional Elements in the Genome (Bing Ren)


Spatial Organization of the Human Genome (Wendy Bickmore)


Chromatin Organization (Susan Gasser)


 
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