A Guide to Target Gene Validation With Quantitative RT-PCR

While next generation sequencing enables researchers to unveil expression levels of the entire genome, qRT-PCR remains the gold standard for measuring transcript levels of individual genes for functional studies and for the purposes of publication. In this webinar, you will learn:• Low (1-5 genes) vs medium (~300 genes) throughput experimental design• Pros and cons of self-designed vs “off the shelf” assays• How to set up your wet lab experiments start to finish• Downloadable example step-by-step experiments with real data analysis and tutorial• Biological considerations (time series data, cell population frequency changes + more)• Examples of these techniques in publications• Common pitfalls and how to avoid them• Limitations of the technique• MIQE and publication standardsWhether you are interested in a few genes or a few hundred, join Matthew Mule as he takes you through the necessary steps to validate expression levels of target genes using qRT-PCR with single gene assays and other medium-throughput platforms.For more information visit: https://bitesizebio.com/webinar/target-gene-validation-quantitative-rt-pcr/