14 Ways to Measure Immune Cell Activation

We’ll discuss specific assays in each of these categories, the joys and pitfalls of each assay, and recommendations on how to choose the best method. You will learn tips and strategies for successful assay development using the following methods:Proliferation:3H-Thymidine UptakeBromodeoxyuridine Uptake (BrdU)ATP LuminescenceFluorescent Dye Reduction (CFSE)Cytokine Measurement:Multiplex vs. Single CytokineChoice of Cytokine (IFNg, TNFa, IL-6, IL-1?, etc.)Kinetics of Cytokine ReleaseSurface Antigen Expression:CD69, CD25, PD-1, etc.Combine with CFSE, Ki67 or BrdUKinetics are ImportantCytotoxicity:Two-Label Flow CytometryCalcein AM Dye ReleaseLuciferase Transduced TargetsAnnexin VActivation of immune cells is the all-important first step in mounting an immune response. Immune cell activation is a popular area of research because so much happens that is key to the downstream goal of fighting infection, cancer, and disease.There are many ways to measure immune cell activation, and they all have utility. Methods can be grouped into four main categories: Proliferation Assays, Cytokine Measurement, Surface Antigen Expression, and Cytotoxicity.For more information visit: https://www.youtube.com/watch?v=ocW-pNVS_gE